Mouse NK2R HEK293
CBP71645
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| I. Background | |
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小鼠 NK2R（Neurokinin-2 Receptor），基因正式名称为 Tacr2（也称 NK2R / TAC2R / SKR），编码速激肽受体家族的 G 蛋白偶联受体，特异性结合内源性配体神经激肽 A（Neurokinin A / Substance K），亲和顺序为 NKA > NKB > SP。 它在小鼠中枢（下丘脑、边缘系统）及外周组织（胃肠道、气道、子宫平滑肌等）广泛表达，顺利获得 Gαq‑PLC‑IP₃‑Ca²⁺ 通路介导平滑肌收缩、神经递质释放、痛觉传递及生殖轴 LH 分泌调节；近年研究还发现下丘脑 NK2R 激活可抑制食欲、增加能量消耗，是肥胖与糖代谢调控的潜在靶点。 |
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| II. Description | |
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Mouse NK2R HEK293 细胞，在 HEK293 细胞的基础上，稳定表达Mouse NK2R。 |
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| III. Introduction | |
| Expressed gene: | Mouse NK2R |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM + 10%FBS + 2μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional assay for Mouse NK2R |
| Transducer: | Gq/Gs |
IV. Representative Data Figure 1. qPCR of Mouse NK2R HEK293.  Figure 2. HTRF cAMP Assay with Mouse NK2R HEK293 (C12) in 500 μM IBMX.  Figure 3. HTRF IP-One Assay with Mouse NK2R HEK293(C12). |
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